The aim of this study was to present a successful model in order to implement knowledge management in physical education faculties of Islamic Azad Universities from organizational culture perspective. The statistical population consisted of the scientific members of physical education faculties of Islamic Azad Universities. The validity of the applied questionnaire was obtained from 14 experts and the reliability was determined by Cronbach's alpha (α=76.6) and then it was distributed among the population. For data analysis, descriptive and inferential statistics were used. One sample t test was used for data analysis and statistical hypothesis testing. Confirmatory factor analysis was used to identify latent variables. To test the hypotheses, the structural equation model by SPSS and LISREL were used. Findings showed that organizational culture highly influenced the success in implementing knowledge management. So, authorities should consider that the organizational culture is very effective for efficient application of knowledge management and they should change or modify undesirable and ineffective indexes through the management of organizational culture change.