The aim of this study was to compare gender identity of male and female professional athletes in Iran. This study was descriptive, correlation and casual-comparative. The population consisted of all male and female, individual and team players in the national and professional sports teams (N=380). Using Morgan and Krejcie Table, 191 male and female athletes in individual and team sports were selected. In 1974, psychologist Sandra Bam provided a test that measured androgyny. The mean feminine identity, as expected, was higher in female athletes (5.11±0.432) and it was lower in male athletes than female athletes (4.96±0.502). Mean androgynous identity was equal in male and female athletes which was 4.95±0.475 in female athletes and 4.93±0.409 in male athletes. t obtained in this test was -2.263 and the significance level was less than 0.05 which showed a significant difference in gender identity between men and women.